Journal: Nature Communications
Article Title: Isolation and characterization of a pangolin-borne HKU4-related coronavirus that potentially infects human-DPP4-transgenic mice
doi: 10.1038/s41467-024-45453-2
Figure Lengend Snippet: a Percentage of body weight changes of infected hDPP4-mice and WT-mice. n = 17 biologically independent animals per genotype. Data are reported as mean ± SD (shown as error bars). The solid dots and lines indicate the weight changes of hDPP4-mice. The hollow dots and dashed lines indicate the weight changes of WT-mice. Orange, live virus-infected mice; blue, heat-inactivated viruses-infected mice; dark gray, cell culture supernatant-infected mice. ANOVA was used for multiple comparisons. b Pathological features of virus-infected hDPP4-mice. H&E-stained lung sections at 3, 6, and 12 DPI are displayed. The hDPP4-mice inoculated with cell medium are used as control. Images are representative of three experimental animals. Original magnification ×200. See also Supplementary Fig. . c Immunohistochemical analysis of lung tissue stained with MAC2 antibody for macrophage detection. The hDPP4-mice inoculated with cell medium are used as control. Images are representative of three experimental animals. Original magnification ×400. See also Supplementary Fig. . d Cytokine response in the lung of hDPP4 (orange) and WT (blue) mice following virus infection. mRNA levels were monitored by qPCR at 1, 3, 6, and 12 DPI. The relative expression of mRNA at 3, 6, and 12 DPI was measured by comparative 2 −∆∆CT method related to 1 DPI and the mean ± SD (shown as error bars) of fold change of three independent biological replicates for each gene at each time point are presented. Student’s T -test (two-tailed) was used for comparisons of hDPP4- and WT-mice. Source data are provided as a Source Data file.
Article Snippet: Pathological examination by IHC staining were conducted with the pangolin-CoV-HKU4-P251T Nucleoprotein Pab (Sino Biological Inc., customized, 1:100 dilution), MAC2 antibody (Cedarlane Laboratories, CL8942AP, 1:1000 dilution), CD3 antibody (Sino Biological Inc., 108567-T08, 1:500 dilution) and CD19 antibody (Cell Signaling Technology, 3574, 1:50 dilution).
Techniques: Infection, Virus, Cell Culture, Staining, Immunohistochemistry, Expressing, Two Tailed Test
